human cpg island microarray kit, 244k (Agilent technologies)
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human cpg island microarray kit, 244k
Human Cpg Island Microarray Kit, 244k, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/244k+microarray/pmc11024764-123-17-18
Average 90 stars, based on 1 article reviews
Human Cpg Island Microarray Kit, 244k, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/244k+microarray/pmc11024764-123-17-18
Average 90 stars, based on 1 article reviews
human cpg island microarray kit, 244k - by Bioz Stars,
2026-09
90/100 stars
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GST Pulldown Assay:Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: .. RNA selectively bound (purple line) to an RBP during a GST-pulldown assay (GST bead is represented as a beige oval) is eluted, directly labeled with either Cy3 or Cy5 (green circles), and hybridized to a Labeling:Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: .. RNA selectively bound (purple line) to an RBP during a GST-pulldown assay (GST bead is represented as a beige oval) is eluted, directly labeled with either Cy3 or Cy5 (green circles), and hybridized to a Microarray:Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: .. RNA selectively bound (purple line) to an RBP during a GST-pulldown assay (GST bead is represented as a beige oval) is eluted, directly labeled with either Cy3 or Cy5 (green circles), and hybridized to a Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: .. Slowly and carefully Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: Using a Tecan HS4800 Pro Hybridization Workstation, wash Agilent 244K microarrays with Pre-hybridization Buffer for 30 seconds (at room temperature), load 120 μL of sample into the microarray sample loading slot, hybridize samples for 20 hours at 42°C, wash for 30 seconds in Hybe Wash Buffer #1, and 30 seconds in Hybe Wash Buffer #2. .. If a Tecan or comparable microarray hybridization workstation is not available, Cy3- and Cy5-labeled RNA samples can be pipetted into a Gasket Slide after which an Article Title: Portraying breast cancers with long noncoding RNAs Article Snippet: .. We also reannotated the Article Title: Structural variation at the CYP2C locus: Characterization of deletion and duplication alleles Article Snippet: Chromosomal Microarray (CMA) Analysis – Replication CMA was performed on the replication cohort using the Affymetrix Cytoscan® HD CNV+SNP array platform (Santa Clara, CA, USA) or the Agilent 244K whole genome oligonucleotide microarray (design 014693; Santa Clara, CA, USA) according to the manufacturer’s instructions. .. The CytoScan® HD microarray contains 1,953,246 non-polymorphic and 743,304 single nucleotide polymorphism (SNP) markers, which are enriched in disease gene areas, and the Article Title: Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency Article Snippet: .. The Kozak sequence variants were synthesised as oligonucleotides on a Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: Agarose , Thermo Scientific (16500100 ). .. Hybridization:Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: .. Slowly and carefully Article Title: RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteins Article Snippet: Using a Tecan HS4800 Pro Hybridization Workstation, wash Agilent 244K microarrays with Pre-hybridization Buffer for 30 seconds (at room temperature), load 120 μL of sample into the microarray sample loading slot, hybridize samples for 20 hours at 42°C, wash for 30 seconds in Hybe Wash Buffer #1, and 30 seconds in Hybe Wash Buffer #2. .. If a Tecan or comparable microarray hybridization workstation is not available, Cy3- and Cy5-labeled RNA samples can be pipetted into a Gasket Slide after which an Sequencing:Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. Article Title: Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency Article Snippet: .. The Kozak sequence variants were synthesised as oligonucleotides on a Immunoprecipitation:Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. Cross-linking Immunoprecipitation:Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. Footprinting:Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. Produced:Article Title: Degenerate consensus sequences in the 3'-untranslated regions of cellular mRNAs as specific motifs potentially involved in the YB-1-mediated packaging of these mRNAs. Article Snippet: The multifunctional protein YB-1 has previously been shown to be the only protein of the cytoplasmic extract of HEK293 cells, which is able to specifically interact with imperfect RNA hairpins containing motifs that are often found in exosomal (e) RNAs.. In addition, it has been revealed that similar hairpins formed by degenerate consensus sequences corresponding to three eRNA-specific motifs are responsible for the cooperative binding of YB-1 to RNA in vitro.. Here, using the photoactivatable-ribonucleoside enhancing cross-linking and immunoprecipitation method applied to HEK293 cells producing FLAG-labeled YB-1, we identified mRNAs cross-linked to YB-1 in vivo and then carried out a search for the aforementioned sequences in the regions of the YB-1 cross-linking sites. |
